DMEM Culture Medium
Characteristic
DMEM medium is a widely used basal medium for supporting the growth of various mammaliancells. Cells successfully cultured in DMEM include primary cells (e.g.,
fibroblasts, neurons, glialcells, HUVECs, smooth muscle cells) and established cell lines such as HeLa, 293, COS-7, and PC.12.
Biologix's DMEM High Glucose Medium is a cell therapy-grade formulation. lt is not onlysuitable for diverse cell culture applications but also extensible to advanced fields
like biopharmaceuticals and cell therapy. Storage conditions: The solution should be stored upon receipt at 2~8ºC . Theprod uct is stable for 12 months.
Features
· Manufactured at a cGMp-compliant facility.
· Sterile filtered by 0.1μm filtration and asepticfilled in a Hundred-stage FFU.
· Each lot of cell culture media issubjected to sterility test, pH, osmolality, endotoxin and cell culturetest.
· Screened through using strict qualitycontrol testing.
Ordering Information
| Cat # |
Product Name
|
Formula
|
Package
|
|
DMEM-500
|
DMEM Culture Medium(1×)
|
[ + ]4.5g/L D-Glucose
[ + ]L-Glutamine
[ + ]110mg/L Sodium Pyruvate
[ + ]Phenol red
[ + ]HEPES
|
500mL/ Bottle, 10 Bottles/Case
|
1.What are the main factors that influence the maximum centrifugal acceleration force?
Use of organic solutions, temperature below 0°C, centrifuging without centrifuge adapter, etc. Conduct a trial run to determine proper conditions before any critical use begins.
The RCF (relative centrifugal force) or G ratings for our disposable centrifuge tubes have been established at room temperature using tubes filled to nominal capacity with water and centrifuging for 30 minutes. recommended carriers, adapters, and cushions that fully support the tubes. If an angle head rotor is used or proper support is not provided, G values will be lower.
Do not confuse speed or revolutions per minute (RPM) with relative centrifugal force (RCF). Instructions for centrifuging a sample at a given RPM and time are incomplete unless the rotor or radius is specified. Protocols should always state the time and RCF value for centrifuging a sample.
2.What are autoclaving guidelines of plastics?
To ensure successful steam sterilization of your plastic labware please adhere to the following guidelines: PLEASE NOTE THAT NOT ALL PRODUCTS ARE AUTOCLAVABLE. Autoclave plastic consumables at 121°C and 15psi/1 atm for 15 minutes. Do not use a dry cycle, as this may cause deformation or weakening of the plastic. Allow temperature within the autoclave to return to 80°C or below before removing products.
3.What are Biologix's Commitments to Quality?
We look at quality from every angle in terms of functionality, safety, purity, formulation, consistency, manufacturing, customer service, technical support, and much more. According to the detailed QA/QC procedures for our manufacturing facility, each and every product is traceable by lot, regardless of packaging format. Products must go through a five-step human inspection before being released for packaging. Biologix applies the most stringent standards to certify that products are free from RNase, DNase, endotoxin (pyrogens), etc. In addition to being ISO 9001:2008, CE compliant, we also make available to our customers lot-specific certificates such as Sterilization Certificates, Nuclease Free Certificates, Certificates of Origin, etc.
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