Hanks Buffer (1×) Balanced Salt Solution in Biological Experiments

• After-sales Service: 5 Years
• Warranty: 5 Years
• Disposable: Non-Disposable
• Model NO.: HANKS-500 HANKS-1000
• Certification: CE, ISO
• Reactant State: Cell
• Material: Buffer
• Sterile: Yes
• Ca2+&Mg2+: No
• Transport Package: Bottled
• Specification: bottled
• Trademark: BIOLOGIX
• Origin: USA

Product Description

Hanks' Buffer is the most commonly used balanced salt solution in biological experiments. It is mainly used for rinsing tissue blocks and cells when collecting samples for cell culture, as well as preparing other reagents. This product is ready-to-use and can be directly used for routine purposes such as washing cells during cell culture after filtration and sterilization. No dilution or filtration sterilization is required before use.

 

Composition

Composition	Concentration (mM)
NaCl	137.93
KCl	5.36
NaHCO3	4.17
KH2PO4	0.44
Na2HPO4	0.34
D-Glucose	5.56

Features

1. Balanced salt solutions commonly used in biological laboratories. 
2. It is one of the commonly used buffers in cell isolation or culture.
3. It has the function of maintaining osmotic pressure, keeping PH stable and providing simple nutrition.
4. After 0.1μm sterilizing filtration and endotoxin detection, it can be directly used for routine cell culture. Endotoxin ensure less than 0.5 EU / mL .
5. Without Ca2+&Mg2+.

Product Nature

PH	7.4±0.1
Phenol red indicator	No phenol red
Sterile	Sterile
Osmotic pressure	270 - 315 mOsm/kg
Endotoxin	<0.5EU/mL

 

Cat #	Product Name	Formula	Package
HANKS-500	Hanks,balanced salt solution(1x)	137.93 mM NaCl, 5.36 mM KCl, 4.17 mM NaHCO3, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose [-]Calcium Chloride [-]Magnesium Chloride	500mL/ Bottle, 10 Bottles/Case
HANKS-1000	Hanks,balanced salt solution(1x)	137.93 mM NaCl, 5.36 mM KCl, 4.17 mM NaHCO3, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose [-]Calcium Chloride [-]Magnesium Chloride	1000mL/ Bottle, 6 Bottles/Case

1. What are the main factors that influence the maximum centrifugal acceleration force?
Use of organic solutions, temperature below 0°C, centrifuging without centrifuge adapter, etc. Conduct a trial run to determine proper conditions before any critical use begins.
The RCF (relative centrifugal force) or G ratings for our disposable centrifuge tubes have been established at room temperature using tubes filled to nominal capacity with water and centrifuging for 30 minutes. recommended carriers, adapters, and cushions that fully support the tubes. If an angle head rotor is used or proper support is not provided, G values will be lower.
Do not confuse speed or revolutions per minute (RPM) with relative centrifugal force (RCF). Instructions for centrifuging a sample at a given RPM and time are incomplete unless the rotor or radius is specified. Protocols should always state the time and RCF value for centrifuging a sample.

2.  What are autoclaving guidelines of plastics?
To ensure successful steam sterilization of your plastic labware please adhere to the following guidelines: PLEASE NOTE THAT NOT ALL PRODUCTS ARE AUTOCLAVABLE. Autoclave plastic consumables at 121°C and 15psi/1 atm for 15 minutes. Do not use a dry cycle, as this may cause deformation or weakening of the plastic. Allow temperature within the autoclave to return to 80°C or below before removing products.

3.  What are Biologix's Commitments to Quality?
We look at quality from every angle in terms of functionality, safety, purity, formulation, consistency, manufacturing, customer service, technical support, and much more. According to the detailed QA/QC procedures for our manufacturing facility, each and every product is traceable by lot, regardless of packaging format. Products must go through a five-step human inspection before being released for packaging. Biologix applies the most stringent standards to certify that products are free from RNase, DNase, endotoxin (pyrogens), etc. In addition to being FDA, ISO 9001:2015, CE compliant, we also make available to our customers lot-specific certificates such as Sterilization Certificates, Nuclease Free Certificates, Certificates of Origin, etc.